This near concordance concerning time details over a 32-fold array implies that Restrict digestion of TF-sure fragments occurs quickly upon addition of Ca++, and demonstrates that digestion time is just not a important parameter.
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Given the benefits that Reduce&Operate provides around ChIP, Skene and Henikoff anticipate that the tactic is going to be viewed as a price-successful and multipurpose different to ChIP. In future, the strategy could be automatic so that a number of analyses can be done without delay.